Features

​• Highly convenient and easy-to-use 2X Master Mix solution: just add your choice of RNA template
​• Proprietary formulation: optimal mix of random primer and oligo-dT
​• Fully optimized reaction system: high sensitivity and high cDNA yield
​• Efficient cDNA target synthesis up to 8 kb in length
​• Broad range of total RNA input capacity (1 ug – 1 pg)
​• Robust and reliable cDNA synthesis system
​• cDNA cloning and sequencing
​• I​​deal for high throughput gene expression analysis

​Description

LeGene cDNA Synthesis Master Mix is a 2X concentrated cDNA synthesis master mix that contains all necessary components for first strand cDNA synthesis including RnaUs-Script reverse transcriptase, RNase inhibitor, primers, dNTPs, Mg2+, and stabilizers (except RNA template).
This cDNA Synthesis Master Mix provides a sensitive and highly convenient pre-mixed cDNA synthesis solution for two-step RT-PCR.
It uses a combination of RnaUs-Script RT and an optimum blend of random primers and oligo-dT to synthesize high yield cDNA from a wide range of RNA input.
This system can detect mRNA targets up to 8 kb. It is ideally suited for high throughput expression profile studies
using both real-time quantitation and end-point RT-PCR. The system contains all the components needed for first-strand cDNA synthesis.
The reagents are sufficient for 50, 200, or 1,000 cDNA synthesis reactions of 20 µl reaction volume each.