Features

​​• Highly convenient and easy-to-use one tube 2X Master Mix solution
​​• An engineered RnaUsScript RT: high yield and broad range of RT reaction temperature (40-55℃)
​​• Broad dynamic range of detection: over six orders of dynamic range
​​• High sensitivity and specificity: detect as low as 0.1pg of total RNA
​​• Antibody based hot start PCR system
​​• Fully optimized qRT-PCR buffer: robustness and reliability
​​• Three choice of master mixes to accommodate the real-time detection systems: No ROX, Low ROX, ROX
​​• Optimally formulated for SYBR-based everyday qRT-PCR
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Description
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LeGene SB-Green One-Step qRT-PCR Master Mix Rox is a 2X concentrated formulation mix. It is designed for the reverse transcription (RT) and polymerase chain reaction (PCR) amplification of a specific target RNA from either total RNA or mRNA in a single tube for the detection and quantification of RNA using real-time detection instruments.
The proprietary 2X qRT-PCR Master Mix contains an engineered RnaUsScript Reverse Transcriptase (RNaseH minus), DnaUs Hot Start Taq DNA polymerase, dNTPs, Mg2+, enhancer, stabilizer and an optimal concentration of ROX for instruments that require ROX for a reference signal. Please consult our website and Instrument Compatibility section below for the correct product for your real-time PCR system.
DnaUs Hot Start Taq DNA polymerase is an antibody-inactivated hot-start enzyme designed to block polymerase activity between ambient to RT reaction temperature. RnaUsScript RT enzyme can synthesize cDNA at a temperature range of 40-55℃. Once the PCR step reaches denaturation temperature (95℃), Taq DNA polymerase activity is restored and the resulting PCR exhibits higher sensitivity, specificity and yield.
The system enables highly sensitive detection from as few as 1 copy of a target gene, with a broad dynamic range that supports accurate quantification of high-copy mRNA from up to 1 μg of total RNA. Sufficient reagents are provided for 50, 200 or 500 amplification reactions of 50 μl each.