Features

​​​• An engineered fusion RT to reduce RNaseH activity and enhance sensitivity, cDNA yield and target length
​​• Synthesize full-length cDNA up to 12 kb
​​• The highest sensitivity: a fusion RT with optimized reaction buffer
​​• Optimal activity at 40-50℃
​​• Robust and high cDNA yield
​​• Ultrapure RT enzyme with consistent performance
​​• Ideal for gene expression study using real-time PCR, cDNA libraries, RT-PCR and sequencing dsDNA

Description

​RnaUsScript-20 reverse transcriptase is a fusion RT, produces higher cDNA yield, and increases RT reaction temperature.
RnaUsScript-20 RT is a single polypeptide chain with a molecular weight of approximately 77 kDa and is a RNA-dependent DNA polymerase that synthesizes the complementary cDNA strand from a single-stranded RNA template to which a primer has been annealed (1,2).
RnaUsScript-20 RT also efficiently extends primers hybridized to single-stranded DNA.
This enzyme is expressed in E. coli (2) and purified to be free of exogenous RNase activity, a common contaminant in commercial preparations of reverse transcriptase. The enzyme is used to synthesize first-strand cDNA up to 12 kb, and 5X cDNA Synthesis buffer is provided.